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human recombinant chimeric fc alk 2 his  (R&D Systems)


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    Structured Review

    R&D Systems human recombinant chimeric fc alk 2 his
    Human Recombinant Chimeric Fc Alk 2 His, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+chimeric+alk+2+fc/Recombinant+Human+ALK-1+Fc+Chimera+Protein%2C+CF/pmc03458350-144-7-14
    Average 93 stars, based on 19 article reviews
    human recombinant chimeric fc alk 2 his - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Regulation of ADMP and BMP2/4/7 at Opposite Embryonic Poles Generates a Self-Regulating Morphogenetic Field
    Article Snippet: After 48 hr, secreted proteins were either dialyzed overnight into PBS for crosslinking experiments or directly immunoprecipitated using anti-Myc beads (Covance). .. Receptor binding assays were carried out as described ( Larrain et al., 2001 ) with Protein A beads (Pierce) using human recombinant chimeric ALK-2-Fc, ALK-3-Fc, ALK-6-Fc, BMPR-II-Fc, mouse Tsg, and Chordin proteins from R&D Systems Inc. Anti-mouse Chordin antibody (R&D Systems, AF758) was diluted 1:500, anti-carboxy-phosphorylated Smad1 antibody (a kind gift from C. Heldin, Ludwig Institute, Uppsala) 1:4000, anti-carboxy-phosphorylated Smad2 antibody (Cell Signaling Technology, #3101) 1:1000, anti-β-catenin antibody (Sigma, C2206) 1:3000, anti-diphosphorylated Erk antibody (Sigma, M8159) 1:1000, and anti-actin antibody (Sigma, A4700) 1:500. .. Antisense MO for Xenopus laevis Admp, Bambi, and Follistatin were obtained from Gene Tools LLC and consisted of the following sequences: Admp MO, 5′-GGTCCATCTCATCAAGCTGCAGCTC-3′; Bambi MO, 5′-GAGATTCCACCATCCAGCCCAGCGT-3′; Follistatin MO, 5′-CATTTAACATCCTCAGTGCTGGGAG-3′.

    Recombinant:

    Article Title: Regulation of ADMP and BMP2/4/7 at Opposite Embryonic Poles Generates a Self-Regulating Morphogenetic Field
    Article Snippet: After 48 hr, secreted proteins were either dialyzed overnight into PBS for crosslinking experiments or directly immunoprecipitated using anti-Myc beads (Covance). .. Receptor binding assays were carried out as described ( Larrain et al., 2001 ) with Protein A beads (Pierce) using human recombinant chimeric ALK-2-Fc, ALK-3-Fc, ALK-6-Fc, BMPR-II-Fc, mouse Tsg, and Chordin proteins from R&D Systems Inc. Anti-mouse Chordin antibody (R&D Systems, AF758) was diluted 1:500, anti-carboxy-phosphorylated Smad1 antibody (a kind gift from C. Heldin, Ludwig Institute, Uppsala) 1:4000, anti-carboxy-phosphorylated Smad2 antibody (Cell Signaling Technology, #3101) 1:1000, anti-β-catenin antibody (Sigma, C2206) 1:3000, anti-diphosphorylated Erk antibody (Sigma, M8159) 1:1000, and anti-actin antibody (Sigma, A4700) 1:500. .. Antisense MO for Xenopus laevis Admp, Bambi, and Follistatin were obtained from Gene Tools LLC and consisted of the following sequences: Admp MO, 5′-GGTCCATCTCATCAAGCTGCAGCTC-3′; Bambi MO, 5′-GAGATTCCACCATCCAGCCCAGCGT-3′; Follistatin MO, 5′-CATTTAACATCCTCAGTGCTGGGAG-3′.



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